Polymerase Chain Reaction (PCR)- ULTRA DETAILED NOTES by Mohd Salman sir | CSIR | GATE | IIT-JAM | MSc | PhD

References : Ecology - Methods in cell biology by marks, Molecular biology techniques by manuels, Biotechnology by david p clarck, etc

Topics covered : PCR introduction and basics, essential features of PCR, nature of primer sequence, degenerate primers, primer length, melting temperature, GC content, annealing temperature, DNA polymerases, DNA pol used in PCR, processivity, fidelity, rate of synthesis, half-life, PYQs, Variations of PCR, RT-PCR, qRT-PCR (TaqMan probe, SYBR green), competitive PCR, Nested PCR, IPCR, touchdown PCR, AP-PCR,RAPD, Anchored PCR, RACE, PCR in genetic engineering, TA-cloning, PCR based site-directed mutagenesis, TAIL-PCR, mrPCR, AFLP, CAPS,GAWTS etc.

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