DNA REPLICATION - ULTRA DETAILED NOTES By Mohd Salman sir | CSIR | GATE | IIT-JAM | MSc | PhD

References : Watson Molecular Biology of the gene, Weaver - molecular Biology, Lewin genes, Alberts, Campbell - Biology. themes in molecular biology.

Topics covered : Components of DNA replication : dNTPs, primer-template junction, bonds in ATP, SN2 reaction, working of DNA polymerase, structure, kinetic proof-reading, Nucleotide Binding Pocket, DNA polymerase : Thumb, finger and palm domain, processive DNA polymerase and Non-processive DNA polymerase, exonuclease proof-reading activity, Drugs that inhibit DNA replication, primer synthesis and removal, action of DNA helicase, mechanism of helicase working, action of SSBPs, action of sliding clamps, how clamp regulate the processivity, loading and unloading of clamp, "Trombone" Model, DNA polymerase III holo-enzyme, eukaryotic DNA polymerases, mode of replication in bacteria and eukarya, replication fork, template dependent and independent DNA polymerases, classification of DNA polymerases, klenow fragment, DNA pol I, III, III, IV, V, DNA pol alpha/primase, topoisomerases (Type I, II, IA, IB), DNA gyrases, okazaki fragment in bacteria and eukarya, DNA Ligases, the replication model, initiator proteins, replicator in E. coli, eukaryotic chromosome replicates exactly once per cell cycle, helicase loading in eukaryotes, OCCM complex, Replication Licensing system (RLS), geminin, mechanism of helicase activation, elongation of replication forks, replisome progression complex (RPC), end replication problem in eukaryotes, protein priming, telomerase, regulation of telomere, sheltrin complex, why telomere protection needed?, t-loop, how does E. coli ensure runaway replication?, FACT (facilitates chromatin transcription), etc.

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